Structural analysis of mono- and bis-sulfated glycosphingolipids by negative liquid secondary ion mass spectrometry with high- and low-energy collision-induced dissociation

✍ Scribed by Keiko Tadano-Aritomi; Harumi Kubo; Philip Ireland; Masaru Okuda; Masaru Okuda; Takeshi Kasama; Shizuo Handa; Ineo Ishizuka

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 599 KB
• Volume: 273
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(95)00106-4

✦ Synopsis

Several underivatized mono-and bis-sulfated glycosphingolipids having gangliotriaose or gangliotetraose core structure were analyzed by negative liquid secondary ion mass spectrometry (LSIMS) with high-and low-energy collision-induced dissociation (CID). In the normal negative I_SIMS spectra, each mono-sulfated glycolipid gave abundant [M -H]-ions and each bis-sulfated glycolipid gave abundant [M + Na -2H]-ions as well as the hydrogen sulfate anion [OSOaH]-. In high-energy CID spectra of the deprotonated molecule, only ions containing a sulfate ester were dearly observed. When a sulfate was present on the non-reducing terminal saccharide residue, a series of ions corresponding to sulfated mono-to tetra-saccharides, resulting from sequential cleavage of glycosidic bonds, were observed. If the sulfate was attached to an internal hexose of the sugar chain, the product ions corresponding to the non-sulfated, non-reducing terminal residue were absent. In contrast, the low-energy CID resulted in extremely simple spectra that contained