Strong uptake of 111in-pentetreotide by an MIBG-negative, xenografted neuroblastoma
✍ Scribed by L. Manil; B. Perdereau; C. Barbaroux; F. Buixy
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 235 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
We investigated the distribution of I I 'In-pentetreotide (Octreoscan, Mallinckraidt) in nude mice xenografted with a human neuroblastoma cell lline (SKIAN, derived from the IAN I line). These cells develop into solid tumours in nude mice and can be grafted repeatedly in grafts of I O8 cells. Animals were sequentially explored by wintigraphy 2, 4, 24 and 48 hr after i.v. injection of 2.5-4 MlBq of the tracer and killed at various times up to 48 hr. '"'In-pentetreotide was rapidly and strongly taken up by all turnours,, with a tumour/muscle (T/M) ratio on resected samples of 20.0 f 5.7 at 2 hr, 23.7 rf: 3.0 at 4 hr, 75.6 f 12.6 at 24 hr and 78.7 2 12.4 at 48 hr, for tumours ranging from 0.5 to 8 g. Scintigriaphy results were quantitatively in agreement. Pre-injection of a 15-20 times larger quantity of unlabelled octreotide s.t. reduced the tumour uptake by a factor of 2. For comparison, nude mice xenografted with the same cell line were also studied with '231-MIBG (4 MBq). At 24 hr, the T/M ratio was 0.62 2 0. C 8. Two other cell lines (glioblastoma ROM and small-cell lung carcinoma SC4 I) which were similarly tested with I ' 'In-pentetreotide (2.54 MBq) gave T / M ratios at 24 hr of 4.8 f 2.8 and 38.4 2: 2 I .8, respectively. Pentetreotide seems to have a high affinity for this MIBG-negative neuroblastoma cell line, which exhibited a clearly higher tumour uptake than the 2 other lines. This work provides new experimental arguments in favour of the particular interest of somatostatin analogues in neuroblastorna and confirms our first clinical results.