Stromal fibroblasts influence oral squamous-cell carcinoma cell interactions with tenascin-C

✍ Scribed by Daniel M. Ramos; Bing L. Chen; Kevin Boylen; Michael Stern; Randall H. Kramer; Dean Sheppard; Stephen L. Nishimura; Deborah Greenspan; Luciano Zardi; Robert Pytela

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: French
• Weight: 247 KB
• Volume: 72
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19970717)72:2<369::aid-ijc28>3.0.co;2-9

✦ Synopsis

In this study we identified tenascin-C (TN-C) and one of its integrin receptors, avb6, in oral squamous-cell carcinoma (SCC) specimens. Neither TN-C nor avb6 are expressed in normal oral mucosa. We also studied 2 human oral squamouscell carcinoma cell lines: the highly invasive HSC-3 cells, and the poorly invasive SCC-25 cells. We determined that adhesion of these cells to TN-C involves both a2 and av integrins. Migration on TN-C by oral SCC cells required fibroblastconditioned medium and did not occur in its absence. This migration was blocked by anti-a2 and anti-av antibodies and was partially inhibited by antibodies to hepatocyte growth factor, epidermal growth factor and transforming growth factor-b1. When seeded on TN-C, the poorly invasive SCC-25 cells formed avb6-positive focal contacts; the HSC-3 cells did not. HSC-3, SCC-25 and PTF cells secrete TN-C into the culture medium, as determined by Western blot. However, when HSC-3 cells were inoculated into the floor of the mouth of nude mice, only murine TN-C could be identified in the reactive stroma adjacent to the resulting tumor nests, demonstrating that in vivo, HSC-3 cells do not secrete TN-C. Our results demonstrate that avb6 and tenascin-C are neoexpressed in oral squamous-cell carcinoma, and that the tumor stromal environment is influential in oral SCC behavior.