Stretching Single DNA Molecules and using High-Speed Camera Power Spectral Analysis to Demonstrate High Force Capabilities of Holographic Optical Tweezers

Stretching single DNA molecules to demon

Stretching single DNA molecules to demonstrate high-force capabilities of holographic optical tweezers

✍ Arnau Farré; Astrid van der Horst; Gerhard A. Blab; Benjamin P. B. Downing; Nanc 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 409 KB

## Abstract The well calibrated force‐extension behaviour of single double‐stranded DNA molecules was used as a standard to investigate the performance of phase‐only holographic optical tweezers at high forces. Specifically, the characteristic overstretch transition at 65 pN was found to appear whe