Stress-related levels of abscisic acid in guard cell protoplasts ofVicia fabaL.
โ Scribed by Elmar W. Weiler; H. Schnabl; C. Hornberg
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 472 KB
- Volume
- 154
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
Levels of abscisis acid (ABA) were determined in isolated guard cell (GCP) and mesophyll cell (MCP) protoplasts of Vicia faba L. in relation to water stress. Incubation of GCP and MCP in 0.4 M or 0.8 M mannitol resulted in an average increase in the level of free abscisic acid (ABA) in the cells of 34% (GCP) and 38% (MCP) within 15-60 rain. It is concluded that guard cell protoplasts form ABA in response to osmotic stress.
๐ SIMILAR VOLUMES
Epidermal peels of Valerianella locusta were acid-treated for 1 h at pH 3.9 to kill all cells other than guard cells. These guard-cell preparations were used to explore the steady-state one-way fluxes and the cytoplasmic and vacuolar contents of abscisic acid (ABA). The method of compartmental analy