Stress proteins are not induced in mammalian cells exposed to radiofrequency or microwave radiation

✍ Scribed by Stephen F. Cleary; Guanghui Cao; Li-Ming Liu; Patsy M. Egle; Keith R. Shelton

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 91 KB
• Volume: 18
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/(sici)1521-186x(1997)18:7<499::aid-bem5>3.0.co;2-y

✦ Synopsis

The induction of stress proteins in HeLa and CHO cells was investigated following a 2 h exposure to radiofrequency (RF) or microwave radiation. Cells were exposed or sham exposed in vitro under isothermal (37 { 0.2 ЊC) conditions. HeLa cells were exposed to 27-or 2450 MHz continuous wave (CW) radiation at a specific absorption rate (SAR) of 25 W/kg. CHO cells were exposed to CW 27 MHz radiation at a SAR of 100 W/kg. Parallel positive control studies included 2 h exposure of HeLa or CHO cells to 40 ЊC or to 45 mM cadmium sulfate. Stress protein induction was assayed 24 h after treatment by electrophoresis of whole-cell extracted protein labeled with [ 35 S]-methionine. Both cell types exhibited well-characterized responses to the positive control stresses. Under these exposure conditions, neither microwave nor RF radiation had a detectable effect on stress protein induction as determined by either comparison of RF-exposed cells with sham-exposed cells or comparison with heat-stressed or Cd // positive control cells.