A flow injection system for assays of total cholesterol and free cholesterol was described. The total cholesterol assay system included an amperometric peroxidase electrode to measure hexacyanoferrate(III) converted from hydrogen peroxide, which was generated by injecting a 2-microliter sample into
โฆ LIBER โฆ
Streptomyces: A superior source for cholesterol oxidase used in serum cholesterol assay
โ Scribed by Porntip H. Lolekha; Yaovalak Jantaveesirirat
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 348 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0887-8013
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โฆ Synopsis
The present study compared three cholesterol oxidase sources (Nocardia, Sreptomyces, and Pseudornonas sps.) for serum cholesterol assay. We found cholesterol oxidase isolated from Streptomyces was superior than those isolated from Nocardia and Pseudomonas sps.
Performances of the reagent contained Streptomyces cholesterol oxidase was excellent and comparable to the performances obtained from reagent contained Nocardia cholesterol oxidase. Moreover, the reagent containing Streptornyces cholesterol oxidase had the lowest cost and had the longest shelf-life
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