Strategy for Low Background-Current Levels in the Electrochemical Biosensors Using Horse-Radish Peroxidase Labels
✍ Scribed by Hyun Ju Kang; Md. Abdul Aziz; Boyoun Jeon; Kyungmin Jo; Haesik Yang
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 548 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1040-0397
No coin nor oath required. For personal study only.
✦ Synopsis
This article describes an electrochemical strategy to achieve low background-current levels in horse-radish peroxidase (HRP)-based electrochemical immunosensors. The strategy consists of (i) the use of an HRP substrate/product redox couple whose formal potential is high and (ii) the use of an electrode that shows moderate electrocatalytic activity for the redox couple. The strategy is proved by a model biosensor using a catechol/o-benzoquinone redox couple and an indium tin oxide (ITO) electrode. The combined effect of high formal potential and moderate electrocatalytic activity allows o-benzoquinone electroreduction with minimal catechol electrooxidation and H 2 O 2 electroreduction. The detection limit for mouse-IgG is 100 pg/mL.