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Strategies for the production of lipase byCandida rugosa; neural estimation of biomass and lipase activity

✍ Scribed by Y. Wang; J. Luopa; T. Rajalahti; S. Linko

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
381 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

Various strategies for the production of lipase by Candida rugosa Diddens and Lodder (ATCC 14830) in stirred tank reactors were investigated. The strain was$rst screened for lipase production with agar plate assay based on the fluorescent dye Rhodamine B, followed by adaptation to the production medium used The highest lipase activity of 7.6 U/ml was obtained on a medium containing 40 g olive oil/l and I g glucose/l, with supplying pure oxygen. Neural networks were used in the estimation of biomass and lipase activity.

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## Abstract A mechanistic model was proposed by Gordillo for the representation of lipase production by __Candida rugosa__, with the bioreactor in batch and fed‐batch operation. However, the model was not able to represent the lipolytic activity. The objective of the present study is to propose an