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Strategies for selecting recombinant CHO cell lines for cGMP manufacturing: Improving the efficiency of cell line generation

โœ Scribed by Alison J. Porter; Andrew J. Racher; Richard Preziosi; Alan J. Dickson

Publisher
American Institute of Chemical Engineers
Year
2010
Tongue
English
Weight
455 KB
Volume
26
Category
Article
ISSN
8756-7938

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In order to establish a mammalian cell expression system with a minimum of selection steps and a stable expression of microgram amounts of recombinant protein (human tissue-type plasminogen activator mutants and chimeric proteins) per 10(6) cells per day, we investigated Chinese hamster ovary cells