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Strategies for folding of affinity tagged proteins using GroEL and osmolytes

✍ Scribed by Hiroo Katayama; Mitchell McGill; Andrew Kearns; Marek Brzozowski; Nicholas Degner; Bliss Harnett; Boris Kornilayev; Dubravka Matković-Čalogović; Todd Holyoak; James P. Calvet; Edward P. Gogol; John Seed; Mark T. Fisher

Book ID
106442428
Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
554 KB
Volume
10
Category
Article
ISSN
1345-711X

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## Abstract A multiple vector system for the intracellular high‐level production of affinity tagged recombinant proteins in __Bacillus megaterium__ was developed. The N‐ and C‐terminal fusion of a protein of interest to a Strep II and a His~6~‐tag is possible. Corresponding genes are expressed unde