𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Strategies for covalent attachment of DNA to beads

✍ Scribed by Gali Steinberg; Katie Stromsborg; Lynette Thomas; David Barker; Chanfeng Zhao

Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
339 KB
Volume
73
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Several covalent attachment chemistries were tested for the immobilization of DNA onto glass beads. The comparison was based on the ability of these chemistries to produce derivatized beads that give good hybridization signals. Cyanuric chloride, isothiocyanate, nitrophenyl chloroformate, and hydrazone chemistries gave us the best (yet comparable) hybridization signals. We further characterized the cyanuric chloride method for the number of attachment sites, number of hybridizable sites, hybridization kinetics, effect of linker length on hybridization intensity and stability of the derivatized beads. Β© 2004 Wiley Periodicals, Inc. Biopolymers, 2004

πŸ“œ SIMILAR VOLUMES

Comparison between Different Strategies
Comparison between Different Strategies of Covalent Attachment of DNA to Glass Surfaces to Build DNA Microarrays
✍ Nathalie Zammatteo; Laurent Jeanmart; Sandrine Hamels; StΓ©phane Courtois; Pierre πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 186 KB

DNA microarray is a powerful tool allowing simultaneous detection of many different target molecules present in a sample. The efficiency of the array depends mainly on the sequence of the capture probes and the way they are attached to the support. The coupling procedure must be quick, covalent, and

Covalent attachment of epoxide hydrolase
Covalent attachment of epoxide hydrolase to dextran
✍ Magda Ibrahim; Patrick Hubert; Edith Dellacherie; Jacques Magdalou; Jocelyne Mul πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 773 KB