Strand specific quantitative real-time PCR to study replication of hepatitis C virus genome
✍ Scribed by Florence Komurian-Pradel; Magali Perret; Birgit Deiman; Mireille Sodoyer; Vincent Lotteau; Glaucia Paranhos-Baccalà; Patrice André
- Book ID
- 113766717
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 65 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0166-0934
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## Abstract The detection of negative‐strand hepatitis C virus (HCV) RNA is a hallmark of replication. A highly sensitive and specific method is required to quantify the very low level of replication inherent to in vitro infection systems. Based on reverse transcription with a tagged primer in the
## Abstract HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand‐specific HCV real‐time RT‐PCR assays were developed and applied to livers explanted because of end‐stage cirrhosis. The assays have broad ranges of dete