Binding of isomaltose and maltose to the
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Masatake Ohnishi; Takashi Matsumoto; Tatsuya Yamanaka; Keitaro Hiromi
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Article
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1990
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Elsevier Science
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English
β 676 KB
The binding of maltose, isomaltose, and D-glucono-1,5-lactone to the glucoamylase [E.C.3.2.1.3] from Aspergillus niger was monitored by the fluorescence-intensity change (delta F) based on the tryptophan residues of the enzyme, and the binding parameters (Kd and delta Fmax) were evaluated from the d