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Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence

✍ Scribed by Montgomery, Jesse L.; Rejali, Nick; Wittwer, Carl T.


Book ID
121741373
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
898 KB
Volume
441
Category
Article
ISSN
0003-2697

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We have developed a simple and sensitive assay method to monitor DNA denaturation. This method is based on (i) incorporation of fluorescent labels onto complementary DNA strands during target-specific amplification by polymerase chain reaction and (ii) detection of DNA denaturation by fluorescence r