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Stoichiometry of estramustine phosphate binding to MAP2 measured by the disassembly of chick brain MAP2: Tubulin microtubules

✍ Scribed by Burns, Roy G.

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 596 KB
Volume: 17
Category: Article
ISSN: 0886-1544
DOI: 10.1002/cm.970170304

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✦ Synopsis

The concentration of estramustine phosphate required to inhibit the assembly or to induce the disassembly of chick brain MAP2:tubulin microtubules is markedly dependent upon the microtubule protein concentration. Analysis of this relationship shows that estramustine phosphate and tubulin compete for common MAP2 sites, that MAP2 can bind 5-6 moles.mole-' estramustine phosphate, and that the K, of these sites is 2 20 p,M estramustine phosphate. It is proposed that two molecules of estramustine phosphate interact with each of the three tubulinbinding sites of MAP2 and inhibit the MAP2:tubulin interaction by neutralising two highly conserved basic residues.

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