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Stoichiometry and Substrate Affinity of the Mannitol Transporter, EnzymeIImtl, from Escherichia coli

✍ Scribed by Veldhuis, Gertjan; Broos, Jaap; Poolman, Bert; Scheek, Ruud M.


Book ID
119919533
Publisher
Biophysical Society
Year
2005
Tongue
English
Weight
187 KB
Volume
89
Category
Article
ISSN
0006-3495

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D-Mannitol is transported and phosphorylated by a specific enzyme I1 of the phosphotransferase system of Escherichia coli. This protein was purified previously in detergent solution and has been partially characterized. As one approach in understanding the structure and mechanism of this enzymeiperm