✦ LIBER ✦

Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy

✍ Scribed by Dr Victor Ionasescu; Lawrence Z. Stern; Rebeca Ionasescu; Peter Rubenstein

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 354 KB
Volume: 5
Category: Article
ISSN: 0364-5134
DOI: 10.1002/ana.410050202

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✦ Synopsis

Abstract

The influence of the membrane‐stablizing agents diphenylhydantoin (DPH) and orgotein (a drug with superoxide dismutase activity) on protein synthesis was studied in cultured human muscle cells obtained from 7 patients with Duchenne muscular dystrophy (DMD) and 14 controls. The cultures were obtained by dissociation and subsequent plating of cells from biopsied quadriceps muscle. These cultures were then labeled with tritiated leucine in the presence and absence of the membrane‐stabilizing drugs. Total protein synthesis (cpm/mg noncollagen protein) in first‐passage muscle cell cultures from DMD patients showed a 35% decrease, but myosin synthesis revealed normal values. DPH and orgotein increased total protein synthesis by 35 and 50%, respectively, in dystrophic cultured cells, but only by 7 and 8%, respectively, in control cultured cells.

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