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Stimulatory effect of zinc on bone formation in tissue culture

โœ Scribed by Masayoshi Yamaguchi; Hidetoshi Oishi; Yasunobu Suketa

Book ID
115779813
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
650 KB
Volume
36
Category
Article
ISSN
0006-2952

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โœฆ Synopsis

The present investigation was undertaken to clarify the in oitro effect of zinc on bone metabolism in tissue culture. Calvaria were removed from weanling rats (3-week-old males) and cultured for periods up to 96 hr in Dulbecco's Modified Eagle Medium (high glucose, 4500 mg/dl) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained lo-' to 10m3 M zinc sulfate. All cultures were incubated at 37" in 5% COr/95% air. Zinc uptake by bone was increased

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Although hydroxyapatite (HAP) and tricalcium phosphate (TCP) are currently used as bone graft substitutes or coatings on metallic prostheses because of their excellent biocompatibility and osteoconductivity, they do not stimulate bone formation or inhibit bone resorption. Zinc, an essential trace el