## Abstract Hamster 4βcell stage embryos were cultured in a proteinβfree, glucoseβfree medium to study the nature of developmental inhibition by inorganic phosphate (Pi). In the absence of Pi, between 40 and 55% of embryos were able to develop to the blastocyst stage but addition of Pi to the mediu
Stimulatory and inhibitory effects of amino acids on the development of hamster eight-cell embryos in vitro
β Scribed by Edward W. Carney; Barry D. Bavister
- Publisher
- Springer US
- Year
- 1987
- Tongue
- English
- Weight
- 543 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1573-7330
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π SIMILAR VOLUMES
Three factors were studied for their effects on the first cleavage division of in vitro fertilized hamster eggs. Eggs from superovulated females were inseminated with precapacitated epididymal hamster sperm in medium containing either fatty acid-free (FAF) or fraction V (V) bovine serum albumin (BSA
The effects of storage of hamster sperm in vitro a t temperatures above freezing on fertilization and early development were studied. Initially, female hamsters woere 5seminated y-tificially with epididymal sperm stored up t o five days at 5 , 16 , or 23-25 C. Eggs were examined at the pronuclear st