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Stimulation of the growth and solamargine production bySolanum paludosummultiple shoot cultures using a new culture medium

✍ Scribed by H. Badaoui; P. Morard; M. Henry

Publisher: Springer
Year: 1996
Tongue: English
Weight: 432 KB
Volume: 45
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00048759

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✦ Synopsis

Organogenic callus cultures of Solanum paludosum were obtained from root, hypocotyle and cotyledon explants of plantlets cultured in sterile conditions. These callus cultures developed multiple shoots which proliferated in Murashige and Skoog basal liquid medium. These multiple shoots produced solamargine, the main steroidal glycoalkaloid present in the unripe fruits.

The optimization of the macronutrient composition of the liquid medium was performed by a method derived from the plant composition. This approach results in the establishment of an appropriate medium (SPOM medium) suitable for the improvement of both growth and solamargine production by multiple shoot cultures ofS. paludosum.

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