𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Stimulation of the collagen α1(I) endogenous gene and transgene in carbon tetrachloride–induced hepatic fibrosis

✍ Scribed by David A. Brenner; Linda Veloz; Rudolf Jaenisch; Joseph M. Alcorn

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
736 KB
Volume
17
Category
Article
ISSN
0270-9139

No coin nor oath required. For personal study only.

✦ Synopsis

Cirrhosis is characterized by a marked increase in the deposition of type I collagen and in the expression of the type I collagen genes d ( 1 ) and &(I). Although al(1) gene regulation has been extensively studied in cultured cells, these results may not be applicable to hepatic fibrogenesis in uiuo. Therefore the regulation of the al(1) endogenous gene and an cul(1) transgene was studied in a transgenic mouse model that has a single copy of a human d ( I ) gene segment containing the structural gene and 1.6 Kb of 5' DNA and 20 Kb of 3' DNA. To initiate hepatic fibrogenesis, we treated mice with the hepatotoxin carbon tetrachloride, either in a single dose or in biweekly doses for a period of 3 to 8 wk. Subsequently, hepatic al(1) messenger RNA levels were determined by a species-specific RNase protection assay. Carbon tetrachloride injections coordinately increased the messenger RNA levels of the cul(1) endogenous gene and the transgene, both immediately and after 8 wk. These experiments demonstrate that this d ( I ) transgene fragment contains information sufficient for appropriate basal and carbon tetrachloride-stimulated hepatic expression. They further demonstrate that sufficient homology exists between the human and mouse regulatory elements for the recognition of human cis-acting elements by mouse trans-acting factors. Thus transgenic mice provide a unique model in which to characterize the collagen cul(1) regulatory elements that are required in uiuo for pathophysiological responses. (HEPATOLOGY 1993; 17:

287-292.)

Cirrhosis represents the final result of complex cellular and biochemical interactions in the liver (1-3). Type I collagen, which is a product of the genes d ( I > and a2(I), becomes the most abundant extracellular matrix

📜 SIMILAR VOLUMES

Gene positional changes relative to the
Gene positional changes relative to the nuclear substructure during carbon tetrachloride-induced hepatic fibrosis in rats
✍ Apolinar Maya-Mendoza; Rolando Hernández-Muñoz; Patricio Gariglio; Armando Arand 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 293 KB 👁 1 views

## Abstract In the interphase nucleus the DNA of higher eukaryotes is organized in loops anchored to a substructure known as the nuclear matrix (NM). The topological relationship between gene sequences located in the DNA loops and the NM appears to be very important for nuclear physiology because p

Increased production of collagen in Vivo
Increased production of collagen in Vivo by hepatocytes and nonparenchymal cells in rats with carbon tetrachloride-induced hepatic fibrosis
✍ Mario Chojkier; Kip D. Lyche; Michael Filip 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 847 KB

We have shown, using the proline:ornithine dual label method, that in normal rats, hepatocytes contribute in vivo about 80 to 90% of the newly synthesized hepatic collagen. In order to quantify the contribution of hepatocytes and nonparenchymal cells to collagen synthesis in vivo in hepatic fibrogen

Hepatic fibrosis in rats produced by car
Hepatic fibrosis in rats produced by carbon tetrachloride and dimethylnitrosamine: Observations suggesting immunoassays of serum for the 75 fragment of type IV collagen are a more sensitive index of liver damage than immunoassays for the NH2-terminal propeptide of type III procollagen
✍ Leena Ala-Kokko; Volkmar Günzler; Jan B. Hoek; Emanuel Rubin; Darwin J. Prockop 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 567 KB

Liver fibrosis was induced in rats both with carbon tetrachloride and dimethylnitrosamine. Assays were performed on steady-state levels of messenger RNAs in the liver for several collagens and basement membrane components. The results indicated marked increases in the steady-state levels of messenge