Stimulation of root and somatic embryo production inEuonymus europaeusL. by an inhibitor of polyamine biosynthesis
✍ Scribed by Laurent Bonneau; Nicole Beranger-Novat; Jeannine Monin; Josette Martin-Tanguy
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 530 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0167-6903
No coin nor oath required. For personal study only.
✦ Synopsis
In vitro formation of roots and somatic embryos is obtained from cotyledon explants of a Spindle tree (Euonymus europaeus L.) cultured on two different media: a medium inducing callus formation and the production of roots, and a medium inducing callus formation, root and somatic embryo production. We studied the effects of CXdifluoromethylomithine (DFMO), a specific, irreversible inhibitor of ornithine decarboxylase (ODC) on root and somatic embryo production, growth and titers of putrescine in Euonymus explants and explant-derived calli. Early changes in putrescine levels were detected in both cultures before the visible emergence of roots or somatic embryos. DFMO rapidly inhibited putrescine accumulation and growth in non-embryogenic calli and highly stimulated rooting activity. DFMO partially inhibited putrescine accumulation in embryogenic calli. This inhibition had no effects on callus growth but significantly reduced the time of emergence of roots and highly stimulated somatic embryo production. The relationship among putrescine, putrescine metabolism, growth, root and somatic embryo formation is discussed.