Stimulation of prostaglandin formation by vasoactive mediators in cultured human endothelial cells
โ Scribed by Francois Alhenc-Gelas; Sue Jean Tsai; Karleen S. Callahan; William B. Campbell; Alice R. Johnson
- Book ID
- 119049317
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 868 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0090-6980
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## Abstract Endothelial cells (EC) line the heart and blood vessels, and they are the principal cellular components of the microvasculature. The presence of circulating platelets is believed to be necessary to maintain the integrity of the capillary endothelium. Growing EC in culture provides an op
## MATERIALS AND METHODS isoprostanes resulting from lipid peroxidation in Materials. [ 3 H]PGE 2 (120 Ci/mmol), [ 3 H]PGF 2a (168 Ci/mmol), and hepatocytes. F 2 -isoprostane release was detected by im-[ 3 H]L-leucine (5 Ci/mmol) were purchased from New England Numunoassay. Conventional cultures o