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Stimulation of liver dna synthesis by a factor from ascites tumor 6c3hed

✍ Scribed by J. W. Yarbro

Book ID
102275954
Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
355 KB
Volume
7
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

C3H mice inoculated with 6C3HED ascites tumor show a gradual increase in the incorporation of ^32^P into liver DNA. Cell‐free supernatant fluid from this tumor has the capacity to stimulate increased incorporation of ^32^P and ^3^H‐labelled thymidine into liver DNA of mice not bearing the tumor. This liver‐stimulating activity (LSA) was destroyed by heating to 60° C for 30 min and by pronase but not by RNase or DNase. The time of maximum stimulation of liver DNA synthesis was on the 4th day after injection of LSA. A 70‐fold purification of LSA was accomplished using sephadex chromatography. It is suggested that LSA is the factor responsible for the increased liver DNA synthesis seen in tumor‐bearing animals, but the mechanism of this action has yet to be explained.

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