Stimulation of intercellular communication of poor-communicating cells by gap-junction-competent cells enhances the HSV-TK/GCV bystander effect in vitro
✍ Scribed by Toshiaki Tanaka; Hiroshi Yamasaki; Marc Mesnil
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- French
- Weight
- 159 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
We have previously shown that gap-junctional intercellular communication (GJIC) appears to play a role in the bystander effect that is observed in anticancer suicide gene therapy mediated by herpes simplex virus (HSV) thymidine kinase (tk) and ganciclovir (GCV). We now report that when connexin-expressing (Cx ؉ ) cells are present within a noncommunicating population of cells (Cx ؊ ), there is GJIC between the Cx ؉ and Cx ؊ cells and that due to this stimulation of GJIC, the bystander effect also occurs when the 2 cell types are mixed. We transfected HeLa cells, which do not express any detectable level of connexin, with Cx43.