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Stimulation of Inositol Phosphate Release in Intact and Permeabilized Fibroblasts

โœ Scribed by GORDON A. JAMIESON JR.; MITCHEL L. VILLEREAL


Book ID
119859543
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
208 KB
Volume
494
Category
Article
ISSN
0890-6564

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Calcium mobilization in permeabilized fi
โœ Leslie L. Muldoon; Gordon A. Jamieson Jr.; Mitchel L. Villereal ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 940 KB

Utilizing a digitonin-permeabilized cell system, we have studied the release of calcium from a non-mitochondria1 intracellular compartment in cultured human fibroblasts (HSWP cells). Addition of 1 mM MgATP to a monolayer of permeabilized cells in a cytosolic media buffered to 150 nM Ca with EGTA rap