Stimulation of human mesenchymal stem cells and osteoblasts activities in vitro on silicon-releasable scaffolds

✍ Scribed by Akiko Obata; Toshihiro Kasuga

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 241 KB
• Volume: 91A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32181

✦ Synopsis

Abstract

Cellular activities of human osteoblasts (HOBs) and mesenchymal stem cells (MSCs) on a silicon‐releasable scaffold, siloxane‐doped poly(lactic acid) and vaterite composite coated with hydroxycarbonate apatite (SPV‐H), were estimated using a medium with or without organic factors, such as dexamethasone (Dex) and β‐glycerophosphate (β‐GP), for inducing mineralization or differentiation. As a control, a composite film containing no silicon (denoted by PV‐H) was prepared using poly(lactic acid) and vaterite. HOBs cultured on SPV‐H formed some agglomerates, bone nodules, after a 21‐day culture in a medium without the organic factors, whereas no agglomerate was observed on PV‐H. Laser Raman spectra implied that calcium phosphate precipitated in HOBs on the SPV‐H. The silicon species in SPV‐H stimulated HOBs to mineralization. The culture tests using MSCs show that the level of alkaline phosphatase (ALP) activity in the cells cultured on SPV‐H increased during the 21‐day culture in a medium without Dex and β‐GP. The level was unchanged in MSCs cultured on PV‐H. In the case of supplementing Dex and β‐GP to the medium, the level of ALP activity in MSCs cultured on SPV‐H was higher than that on PV‐H at all time points during the 21‐day culture. The silicon species in SPV‐H were regarded to induce and enhance the osteogenic differentiation of MSCs. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009