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Stimulation of glioma-cell migration by laminin and inhibition by anti-α3 and anti-β1 integrin antibodies

✍ Scribed by Berit B. Tysnes; Lone F. Larsen; Gro O. Ness; Rupavathana Mahesparan; Klaus Edvardsen; Inmaculada Garcia-Cabrera; Rolf Bjerkvig

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
726 KB
Volume
67
Category
Article
ISSN
0020-7136

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✦ Synopsis

An induction of laminin in the confrontation zone between tumor cells and normal brain tissue has been observed in our model systems in vivo and in vitro. In order to study the effects of ECM components on glioma-cell migration and invasion, we have used 2 lacZ-transfected glioma cell lines, AN I //acZ and U-25 I /lacZ. Cell migration from multicellular spheroids was studied using different types of media: DMEM with 10% serum, Ultra Culture medium, and filtrated DMEM with serum in which the protein fraction > I00 kDa had been removed by ultrafiltration. Laminin, fibronectin and collagen type-IV were individually added to the different media, and cell migration from the spheroids was studied. The results show that cell migration in both cell lines, was stimulated by laminin and fibronectin. Collagen type-IV stimulated only cell migration of U-25 I /lacZ cells. Scanning electron microscopy revealed an extensive change in cell shape as a result of laminin stimulation. Flowcytometric studies showed that both AN I /lacZ and U-25 I /lacZ strongly express the a3p I integrin receptor, which can bind to several ECM components (laminin. fibronectin, collagen). Immunofluorescence microscopy demonstrated that the same integrin sub-units were expressed in multicellular spheroids. When monoclonal antibodies to a3 and p I were added to the lamininstimulated cultures, cell migration was significantly reduced. This indicates that the a3p I integrin receptor plays an important role during glioma-cell migration.

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