Stimulation of enzymes involved in tylosin biosynthesis by cyclic feeding profiles in fed batch cultures

✍ Scribed by K. Vu-Trong; P. P. Gray

• Publisher: Springer Netherlands
• Year: 1984
• Tongue: English
• Weight: 295 KB
• Volume: 6
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00129305

✦ Synopsis

Previous reports have shown that feeding glucose and monosodium glutamate in a cyclic fashion to S treptomyces fradiae fermentations greatly stimulated the specific production rate for tylosin (qty!osin) over that which could be obtained in fermentations where the nutrients were fed at a constant rate, all other conditions being the same. In this report it was shown that the activities of three of the enzymes involved in tylosin biosynthesis, propionyl-coenzyme A carboxylase (EC 6.4.1.3), methylmalonyl-coenzyme A carboxyltransferase (EC 2.1.3.1) and macrocin 3'-0-methyltransferase, were stimulated in the case of the cyclic fed-batch fermentation when compared with control levels i.e. the cyclic feed stimulated the activities of enzymes on the tylosin pathway as well as the qtylosin values.