Stimulation of berberine secretion and growth in cell cultures ofThalictrum minus

✍ Scribed by Daniel D. Smolko; Steven W. Peretti

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 704 KB
• Volume: 14-14
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00233776

✦ Synopsis

An endo-pectate lyase (PL; EC 4.2.2.2), originally cloned fiom the phytopathogenic bacterium Erwinia chrysanthemi EC16, was expressed in recA (-) E. coli strain DK1, purified to a single band by isoelectric focusing and used to induce berberine production in established plant suspension cultures of Thalictrum minus L. subsp. saxatile. Addition of 10(-9)M pectate lyase c (PLc) stimulated berberine production and enhanced secretion of the alkaloid into the medium. A lower concentration of PLc, 10(-11)M, stimulated a transient two-fold increase in cell growth rate relative to untreated cultures. Parallel changes in L-phenylalanine ammonia lyase (PAL; EC 4.3.1.5) activity with the rate of berberine synthesis and the inverse relationship between cell growth and berberine synthesis imply that berberine synthesis is stress-related in this cell line.