𝔖 Bobbio Scriptorium
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Stimulation of ATPase activity of 30-S dynein with microtubular protein

✍ Scribed by Minoru Otokawa

Book ID
115715944
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
147 KB
Volume
275
Category
Article
ISSN
0005-2728

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πŸ“œ SIMILAR VOLUMES

Heterogeneity of 14S and 30S dynein ATPa
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✍ Jacob J. Blum; A. Hayes; T. Vanaman; F. H. Schachat πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 English βš– 744 KB

## Abstract Demembranated cilia of Tetrahymena pyriformis were extracted with KCl or Tris‐EDTA and the crude dyneins from each resolved by sucrose density gradient sedimentation into 14S‐K, 30S‐K, 14S‐E and 30S‐E dyneins, respectively. The calmodulin activation ratio (ATPase activity in presence of

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Cilia from the protozoan Tetrahymena pyriformis were demembranated and then extracted for 5 min with a buffer containing 0.5 M NaCl. The briefly extracted axonemal pellet was then reextracted for about 20 hr. The soluble material obtained from each extraction was resolved into 14S and 30S dynein ATP