Stimulation by thyroid hormone of phosphate transport in primary cultured renal cells

✍ Scribed by Lalita Noronha-Blob; Valerie Lowe; Bertram Sacktor

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 781 KB
• Volume: 137
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041370111

✦ Synopsis

The regulation by thyroid hormone of phosphate transport in primary cultured chick renal cells was examined. The more physiologically active L- analogs of triiodothyronine and thyroxine, but not the D-analogs of the hormones, stimulated the Na+-dependent phosphate uptake system. Na+-independent phosphate uptake and Na+-dependent uptakes of a-methylglucoside and L-proline were unaffected. The increase in Na+-dependent phosphate uptake was concentration dependent, exhibited an induction period, and was blocked by inhibitors of RNA and protein synthesis. The stimulation of phosphate uptake by triiodothyronine wa5 due to an increased V, , , rather than to an altered affinity for phosphate. These findings demonstrate that thyroid hormone acts directly o n renal cells to modulate phosphate transport and suggest that the renal cell system may serve as a model to examine the mechanism by which thyroid hormone controls gene expression and regulates plasma membrane transport function.