Changes in intracellular ion concentrations have been correlated with the activation of an endogenous endonuclease and thus internucleosomal DNA cleavage during apoptosis in many cell types. We investigated whether intracellular pH could play a significant role in apoptotic initiation and progressio
✦ LIBER ✦
Stimulation by serum of the Na+/H+ antiporter in quiescent pig kidney epithelial (LLC-PK1) cells and role of the antiporter in the reinitiation of DNA synthesis
✍ Scribed by John G. Haggerty; Neeraj Agarwal; Dr. Kurt Amsler; Carolyn W. Slayman; Edward A. Adelberg
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 514 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Measurement of [3H]TdR incorporation
Cumulative tritiated thymidine Q3H]TdR) incorporation was assayed as described by Amsler and Cook (1982) using 5 pM [3H]TdR (0.2 pCi/ml) in a-MEM medium. For the pulse labeling shown in Figure 2A, the activity
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## Abstract The present study was performed to investigate the regulation of cytosolic pH (pHi) and DNA synthesis by parathyroid hormone(PTH) and PTH‐related peptide (PTHrP) in osteoblasts, using osteoblastic osteosarcoma cells, UMR‐106 which possessed PTH‐responsive dual signal transduction system