Using a modification of the agar gel method for bone marrow culture, serum from various strains of mice has been tested for colony stimulating activity. Ninety percent of sera from AKR mice with spontaneous or transplanted lymphoid leukemia and 40-50% of sera from normal or preleukemic AKR mice sti
Stimulation by Leukaemic Sera of Colony Formation in Solid Agar Cultures by Proliferation of Mouse Bone Marrow Cells
β Scribed by BRADLEY, T. R.; METCALF, D.; ROBINSON, W.
- Book ID
- 109655808
- Publisher
- Nature Publishing Group
- Year
- 1967
- Tongue
- English
- Weight
- 247 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/213926a0
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## Abstract An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures __in vitro__. Colony formation was shown to be related to the concentration and not the total amount o
## Abstract Medium harvested from cultures of mouse Lβcells contains βconditioning factor activityβ (CFA) that may be detected by its ability to stimulate colony formation by mouse marrow cells in culture. The active material has been characterized and appears to be a glycoprotein with properties s