Stiffness of γ subunit of F1-ATPase
✍ Scribed by Daichi Okuno; Ryota Iino; Hiroyuki Noji
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 562 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1432-1017
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## Abstract The yeast __Saccharomyces cerevisiae__ F~1~F~O~‐ATPase ε‐subunit (61 residues) was synthesized by the solid‐phase peptide approach under both acidic and basic strategies. Only the latter strategy allowed us to obtain a pure ε‐subunit. The strong propensity of the protein to produce few
The combined use of proteolytic digestion and lactoperoxidase catalyzed labelling with [125I] applied to membrane-bound or soluble pure F1-ATPase from Micrococcus lysodeikticus has allowed us to establish the topography of its alpha, beta, gamma and delta subunits within the protein molecule and wit