✦ LIBER ✦
Sterol mutants of Chlamydomonas reinhardtii: Characterisation of three strains deficient in C24(28) reductase
✍ Scribed by Ekaterina Salimova; Arminio Boschetti; Waldemar Eichenberger; Ludmila Lutova
- Book ID
- 104371579
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 123 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0981-9428
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✦ Synopsis
Three mutants of Chlamydomonas reinhardtii (strain arg7cw15) were obtained using the strategy of insertional mutagenesis by random plasmid integration with subsequent selection for resistance against the polyene antibiotic nystatin. Sterols were isolated by precipitation with digitonin, fractionated by both normal and argentation TLC, and then analysed by