Steroid Receptor Methods: Protocols and Assays

✍ Scribed by Mark Danielsen (auth.), Benjamin A. Lieberman (eds.)

• Publisher: Humana Press
• Year: 2001
• Tongue: English
• Leaves: 397
• Series: Methods in Molecular Biology 176
• Edition: 1
• Category: Library

✦ Synopsis

Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In Steroid Receptor Methods: Protocols and Assays, a distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA-binding characteristics and hormone-binding assays and with the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness. Finally, a discussion by example presents the most current bioinformatics tools and analytical techniques.
Timely and highly accessible, Steroid Receptor Methods: Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery.

✦ Table of Contents

Front Matter....Pages i-xiv
Front Matter....Pages 1-36
Front Matter....Pages 3-22
Front Matter....Pages 23-36
Front Matter....Pages 37-129
Front Matter....Pages 39-54
Front Matter....Pages 55-65
Back Matter....Pages 67-79
....Pages 81-90

✦ Subjects

Biochemistry, general