✦ LIBER ✦
Stereoselective synthesis of L-[15N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system
✍ Scribed by Maria Chiriac; Iulia Lupan; N. Bucurenci; O. Popescu; N. Palibroda
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- French
- Weight
- 106 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
We have developed an efficient stereospecific enzymatic synthesis of L‐[^15^N]‐valine, L‐[^15^N]‐leucine, L‐[^15^N]‐norvaline, L‐[^15^N]‐norleucine and L‐[^15^N]‐isoleucine from the corresponding α‐keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L‐amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1–10 g). Copyright © 2008 John Wiley & Sons, Ltd.