Stereoselective interaction of an azole antifungal agent with its target, lanosterol 14α-demethylase (cytochrome p-45014dm): A model study with stereoisomers of triadimenol and purified cytochrome p-45014dm from yeast

Characteristics of the heterologously ex

Characteristics of the heterologously expressed human lanosterol 14α-demethylase (other names: P45014DM, CYP51, P45051) and inhibition of the purified human andCandida albicans CYP51 with azole antifungal agents

✍ Lamb, David C.; Kelly, Diane E.; Waterman, Michael R.; Stromstedt, Maria; Rozman 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 192 KB

Human and Candida albicans CYP51 were purified to homogeneity after GAL10-based heterologous expression in yeast in order to resolve the basis for the selective inhibition of the fungal enzyme over the human orthologue by the azole drugs ketoconazole and itraconazole, used in the treatment of system