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Stepwise kinetic equilibrium models of quantitative polymerase chain reaction

✍ Scribed by Gary Cobbs

Book ID
119894212
Publisher
BioMed Central
Year
2012
Tongue
English
Weight
595 KB
Volume
13
Category
Article
ISSN
1471-2105

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Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference