Step-change versus gradient lithium buffers for single-column chromatography of complex amino acid mixtures

✍ Scribed by J.L. Young; M. Yamamoto

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 657 KB
• Volume: 78
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)99059-4

✦ Synopsis

Separation of more than Afty acidic, neutral, ancl basic amino acids, including asparagine and glutamine W;LS achieved on a single Go x 0.9 cm column of spherical resin using lithium citrate hi'fcrs. Usefully improved resolution results from a gradient VS. a step-change buffer system. Some limitations of tllc methods cspccially with cotnplcs natural samples having numerous unknowns and variable concentrations, "%.n r.r\t,c;rlnwa~ T;lw*