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Step-by-step Control by Time-of-flight Secondary Ion Mass Spectrometry of a Peptide Synthesis Carried Out on Polymer Beads

✍ Scribed by C. Drouot; C. Enjalbal; P. Fulcrand; J. Martinez; J. L. Aubagnac; R. Combarieu; Y. de Puydt

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 252 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199609)10:12<1509::aid-rcm663>3.0.co;2-g

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✦ Synopsis

The use of time-of-flight secondary ion mass spectrometry (Tof-SIMS) for the step-by-step control of a peptide synthesis on a solid support is illustrated. The positive secondary ions, issuing from energetic fragmentation, are characteristic of the terminal groups of the step under investigation. The negative secondary ions result from the cleavage of the benzyl ester link between the polymer support and the first amino acid, and provide direct identification of the growing sequence. Tof-SIMS offers this unique analytical possibility without any preliminary separation of the peptide from its support.

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