Stem Cell Mobilization: Methods and Protocols

✍ Scribed by Gerd Klein, Patrick Wuchter

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 250
• Series: Methods in Molecular Biology 2017
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.

✦ Table of Contents

Front Matter ....Pages i-xi
Evolution of Peripheral Blood Stem Cell Transplantation (Anthony D. Ho)....Pages 1-10
In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents (Motomu Tanaka)....Pages 11-27
Colony Formation: An Assay of Hematopoietic Progenitor Cells (Romy Kronstein-Wiedemann, Torsten Tonn)....Pages 29-40
Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarking (Katharina Kriegsmann, Patrick Wuchter)....Pages 41-58
Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cells and Niche Cells (Reinhard Henschler, Rudolf Richter)....Pages 59-70
Microfluidic Shear Force Assay to Determine Cell Adhesion Forces (Julia Hümmer, Julian Koc, Axel Rosenhahn, Cornelia Lee-Thedieck)....Pages 71-84
A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche (Eric Gottwald, Cordula Nies, Patrick Wuchter, Rainer Saffrich, Roman Truckenmüller, Stefan Giselbrecht)....Pages 85-95
Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant (Sabrina Zippel, Annamarija Raic, Cornelia Lee-Thedieck)....Pages 97-107
Intravital Imaging of Blood Flow and HSPC Homing in Bone Marrow Microvessels (Jonas Stewen, Maria Gabriele Bixel)....Pages 109-121
Assessing Cellular Hypoxic Status In Situ Within the Bone Marrow Microenvironment (Ute Suessbier, César Nombela-Arrieta)....Pages 123-134
Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry (Angela Jacobi, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, Jochen Guck)....Pages 135-148
Assessment of Proteolytic Activities in the Bone Marrow Microenvironment (Andreas Maurer, Gerd Klein, Nicole D. Staudt)....Pages 149-163
Analysis of the Complement Cascade Activation During Mobilization of Hematopoietic Stem/Progenitor Cells (Anna I. Grabowska, Jakub M. Hawryluk)....Pages 165-175
A Freezing Protocol for Hematopoietic Stem Cells (Petra Pavel, Sascha Laier)....Pages 177-192
Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays (Yu Wei Zhang, Nina Cabezas-Wallscheid)....Pages 193-203
Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells (Martina Konantz, Joëlle S. Müller, Claudia Lengerke)....Pages 205-217
Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells (Walter de Back, Thomas Zerjatke, Ingo Roeder)....Pages 219-243
Back Matter ....Pages 245-248

✦ Subjects

Life Sciences; Stem Cells