Steady-state kinetic studies of arginase with an improved direct spectrophotometric assay

✍ Scribed by C.Nick Pace; Andres Buonanno; Jeannie Simmons-Hansen

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 361 KB
• Volume: 109
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90646-6

✦ Synopsis

A direct spectrophotometric assay for arginase developed by R. L. Ward and P. A. Srere t 1967. At~crl. Biochem. 18, 102) is extended so that it can be used at pH 7.5 or 9.5. at higher substrate concentrations, for assaying crude sources of arginase. and for steady-state kinetic studies of the enzyme. Steady-state kinetic studies of beef and rat liver arginase are reported and compared with Gmilar results obtained using other assay procedures. A number of problems encountered in steady-state kinetic studies of argina\e are discussed.