The graphical analysis of steady-state kinetic data according to Eadie-Augustinsson-Hofstee (EAH plot) is illustrated for multisubstrate systems and compared with the double reciprocal plot (Lineweaver-Burk plot) commonly used. It is emphasized that the choice of graphical representation may be of c
Steady state enzyme kinetics: Experimental design and data analysis by microcomputer
β Scribed by Billy D. Roberts; Kurt E. Ebner
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 142 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0020-7101
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π SIMILAR VOLUMES
A special mixing device for initiating enzyme-catalyzed reactions is used to rapidly achieve an unperturbed quasi-steady state. An on-line computer is employed to sample the initial conditions, the mixing time, and concentrations that change as a function of time during this quasi-steady state phase
The steady-state velocity equation is derived for the general reaction scheme containing n kinds of enzyme species connected by a network of reversible reaction steps. The general equation is represented by the net rate constants as well as the true rate constants of the individual reaction steps. U