Status of glutathione and other thiols and disulfides in human plasma
β Scribed by Wayne A Kleinman; John P Richie Jr.
- Book ID
- 113992277
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 386 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0006-2952
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A simple and sensitive HPLC method is proposed for the determination of glutathione (GSH) in human mononuclear cells, based on the derivatization of the tripeptide with Ellman's reagent. The mobile phase was composed of a mixture of methanol and ammonium formate (10:90 v/v, with a flow rate of 1 mL/
A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 related sulfur-containing amino acids or their derivatives has been described. The procedure is based upon the i