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Status and expression of the p16INK4 gene in human thyroid tumors and thyroid-tumor cell lines

✍ Scribed by Viola Calabrò; Maria Strazzullo; Girolama La Mantia; Monica Fedele; Christian Paulin; Alfredo Fusco; Luigi Lania

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
648 KB
Volume
67
Category
Article
ISSN
0020-7136

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✦ Synopsis

The p 16INK4 tumor-suppressor gene (also known as CDKNZ, CDK41 and MTSI) encodes a negative regulator of the cell cycle. This gene, located in 9pZ I, is mutated or homozygously deleted in a high percentage of tumor cell lines and specific types of primary tumors. We have examined the status of the p16INK4 gene in 31 thyroid tumors and 7 thyroid cell lines. No DNA abnormalities were found in primary tumors. Conversely, p16INK4 gene structural alterations, deletions and point mutations were found in 4 thyroid cell lines. The expression of the 2 different p16lNK4 mRNAs, the p Ibo! and p 168 transcripts, was determined by RNA-PCR experiments. All the primary thyroid tumors expressed the 8 transcript, while the p I6a was barely detectable. The thyroid cell lines always expressed the p168 transcript, while the o! transcript was absent or, whenever present, coded for a mutated form of the p16INK4 gene roduct. Taken directly involved in the process of thyroid-tumor development, but it probably gives cells in tissue culture a selective growth advantage. o 1996 Wilq-Liss, Inc. together, our results suggest that loss of pl6INK . Y function is not

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