Statistical optimization of a reversed-phase ion-pair liquid chromatographic method for the analysis of tolmetin sodium in dosage forms

✍ Scribed by Rona Stromberg

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 573 KB
• Volume: 448
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84561-1

✦ Synopsis

A quantitative liquid chromatographic method in which tolmetin sodium is separated from an internal standard on a C18 column with detection at 317 nm has been developed with the aid of two statistical optimization procedures. The effects of simultaneously varying the pH, methanol-water ratio, and the concentrations of buffer and ion-pair reagent in the mobile phase were studied. A two-level factorial design was used to investigate the interactions among the variables, and the sequential simplex procedure was used to optimize the separation. A novel quality criterion was developed for the simplex optimization. Using synthetic mixtures, the mean recovery value +/- S.D. (n = 6) of tolmetin sodium was 98.7 +/- 0.19% for tablets and 98.5 +/- 0.12% for capsules. The assay results for commercial tablets and capsules were comparable to those obtained by the USP XXI procedure.