Statistical mechanics of noncovalent bonds in polyamino acids. VII. Fluorescence as an indication of conformation

✍ Scribed by Douglas C. Poland; Harold A. Scheraga

• Publisher: Wiley (John Wiley & Sons)
• Year: 1965
• Tongue: English
• Weight: 451 KB
• Volume: 3
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1965.360030403

✦ Synopsis

Synopsis

I t is shos n how the technique of fluorewetice may be med to abbess conclusions about conformation which are based on a statistical mechanical treatmelit of simple protein models. Specifically, the state of bonding and distance between two chromophores in a molecule depend on the overall conformation, which can be computed for model systems. On the assumption that the intensity and polarization of fluorescence are affected by exciton transfer between the chromophores, it is possible to compute the effect of conformation on fluorescence. It is demonstrated that the conformational changes computed in the preceding paper will give rise to marked changes in the intensity and polarization of fluorescence.